【貨號】 BP-DL023

【規(guī)格】 7×50mL/7×100mL

【保存】 10~30℃，避光，12個月有效。

【產(chǎn)品組成】

  

【產(chǎn)品簡介】

結(jié)締組織狹義上是指其含有的三種纖維：膠原纖維、網(wǎng)狀纖維、彈力纖維，而膠原纖維（collagenfiber）是分布最廣、含量最多的一種纖維。Masson三色染色又稱馬松染色，是結(jié)締組織染色中最經(jīng)典的一種方法，是膠原纖維染色權(quán)威而經(jīng)典的技術(shù)方法。所謂三色染色通常是指染胞核和能選擇性的顯示膠原纖維和肌纖維。該法染色原理不陰離子染料分子的大小和組織的滲透有關(guān)：分子的大小由分子量來體現(xiàn)，小分子量易穿透結(jié)構(gòu)致密、滲透性低的組織；而大分子量則只能進(jìn)入結(jié)構(gòu)疏松的、滲透性高的組織。然而，淡綠或苯胺藍(lán)的分子量都很大，因此Masson染色后肌纖維呈紅色，膠原纖維呈綠色（淡綠）或藍(lán)色（苯胺藍(lán)），主要用于區(qū)分膠原纖維和肌纖維。染色穩(wěn)定；分化時間短，1~2s；色彩清晰鮮艷；適用范圍廣，適宜于組織的石蠟切片、冰凍切片等染色；所染切片保存時間長且不易褪色。

【使用方法】 

1、切片常規(guī)脫蠟至水，用配制好的Weigert鐵蘇木素染色5~10min。

2、用酸性乙醇分化液分化，水洗。

3、用Masson藍(lán)化液返藍(lán)，水洗。

4、蒸餾水洗1min。

5、麗春紅品紅染色液染色5~10min。

6、在上述操作過程中按蒸餾水:弱酸溶液=2:1比例配制弱酸工作液，用弱酸工作液洗1min。

7、磷鉬酸溶液洗1~2min。

8、用配制好的弱酸工作液洗1min。

9、直接入固綠染色液中染色1~2min。

10、用配制好的弱酸工作液洗1min。

11、95%乙醇快速脫水。無水乙醇脫水3次，每次5~10s。

12、二甲苯透明3次，每次1~2min。中性樹膠封固。

【染色結(jié)果】

  

【注意事項】

1、切片脫蠟應(yīng)盡量干凈。固定起著重要的作用，使用不同的固定液可延或縮短染色時間。

2、取A1、A2等量混合即為Weigert鐵蘇木素染液，一般24h失去染色力。

3、酸性乙醇分化時間應(yīng)根據(jù)切片厚薄、組織的類別和新舊而定。

4、弱酸溶液可使色彩更清晰鮮艷，如使用量大可自行配制0.1~0.3%乙酸溶液予以替代。

5、磷鉬酸分化時要在鏡下控制，分化到膠原纖維呈淡紅色、纖維呈紅色即可。分化時間根據(jù)染色深淺而定，一般1~2min。

6、Masson藍(lán)化液亦可自行配制Scott促藍(lán)液或0.1~1%碳酸鋰水溶液予以替代。

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